control empty vector plvx ires zsgreen1 Search Results


michael davidson  (Addgene inc)
93
Addgene inc michael davidson
Michael Davidson, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+vector+plvx+ires+zsgreen1/ZsGreen1-H2B-6+(Plasmid+%2356514)/bio_rxiv__2025__11__17__683537-47-7-9
Average 93 stars, based on 1 article reviews
michael davidson - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
pef1a dcas9 cbio ires zsgreen1 puro vector  (Addgene inc)
92
Addgene inc pef1a dcas9 cbio ires zsgreen1 puro vector
a Reduced expression level of Mbd1 was revealed by RNA-seq from two independent experiments and Western blot. β-actin was used for a Western blotting control. b Chromatin occupancy of CTCF and Rad21 in parental and Matr3 KO cells near Mbd1 locus is shown. c CAPTURE experiment was performed using sgRNAs directed to a putative cis-regulatory element near Mbd1 which is occupied by CTCF and cohesin, and an adjacent upstream, as a negative control (NC) (Figs. 5b and S4b). d Purification of biotinylated <t>dCas9</t> and Western blot indicated that Matr3 interacted with the putative element (Mbd1) but not with the upstream control (NC). Relative enrichment of Matr3 protein at the putative element to the upstream control was quantified from three independent experiments. e Radial location of the Mbd1 gene was observed by 3D DNA FISH. For quantification, the nuclear radius was divided into five shells and the number of signals in each shell was counted. The graph is the result of 2–3 independent experiments and the change was significant under a two-sided t test ( p = 0.006; Cohen’s d = 0.32). f Snapshot of Hi-C data at the Mbd1 locus showing compartments in parental and Matr3 KO cells and the difference in insulation score (parental in green and Matr3 KO in magenta). g , h Deletion of CTCF/cohesin binding region (shaded box in Fig. 5b) by CRISPR/Cas9 reduced Mbd1 expression. RNA ( g ) and protein ( h ) levels were examined by RT-PCR ( n = 3) and Western blotting, and compared to Mbd1 KO cells. β-actin was used for a Western blot control. Data were the result of 2–3 independent experiments and error bars represent mean ± s.d. Source data are provided as a Source Data file.
Pef1a Dcas9 Cbio Ires Zsgreen1 Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+vector+plvx+ires+zsgreen1/CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1+(Plasmid+%23138418)/pmc08556400-579-3-12
Average 92 stars, based on 1 article reviews
pef1a dcas9 cbio ires zsgreen1 puro vector - by Bioz Stars, 2026-10
92/100 stars
  Buy from Supplier
circular sgrna vector plasmid  (Addgene inc)
93
Addgene inc circular sgrna vector plasmid
a Reduced expression level of Mbd1 was revealed by RNA-seq from two independent experiments and Western blot. β-actin was used for a Western blotting control. b Chromatin occupancy of CTCF and Rad21 in parental and Matr3 KO cells near Mbd1 locus is shown. c CAPTURE experiment was performed using sgRNAs directed to a putative cis-regulatory element near Mbd1 which is occupied by CTCF and cohesin, and an adjacent upstream, as a negative control (NC) (Figs. 5b and S4b). d Purification of biotinylated <t>dCas9</t> and Western blot indicated that Matr3 interacted with the putative element (Mbd1) but not with the upstream control (NC). Relative enrichment of Matr3 protein at the putative element to the upstream control was quantified from three independent experiments. e Radial location of the Mbd1 gene was observed by 3D DNA FISH. For quantification, the nuclear radius was divided into five shells and the number of signals in each shell was counted. The graph is the result of 2–3 independent experiments and the change was significant under a two-sided t test ( p = 0.006; Cohen’s d = 0.32). f Snapshot of Hi-C data at the Mbd1 locus showing compartments in parental and Matr3 KO cells and the difference in insulation score (parental in green and Matr3 KO in magenta). g , h Deletion of CTCF/cohesin binding region (shaded box in Fig. 5b) by CRISPR/Cas9 reduced Mbd1 expression. RNA ( g ) and protein ( h ) levels were examined by RT-PCR ( n = 3) and Western blotting, and compared to Mbd1 KO cells. β-actin was used for a Western blot control. Data were the result of 2–3 independent experiments and error bars represent mean ± s.d. Source data are provided as a Source Data file.
Circular Sgrna Vector Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+vector+plvx+ires+zsgreen1/Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1+(Plasmid+%23138461)/pm39894446-131-17-21
Average 93 stars, based on 1 article reviews
circular sgrna vector plasmid - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
plvx-mcmv-zsgreen1  (Biowit Technologies)
90
Biowit Technologies plvx-mcmv-zsgreen1
a Reduced expression level of Mbd1 was revealed by RNA-seq from two independent experiments and Western blot. β-actin was used for a Western blotting control. b Chromatin occupancy of CTCF and Rad21 in parental and Matr3 KO cells near Mbd1 locus is shown. c CAPTURE experiment was performed using sgRNAs directed to a putative cis-regulatory element near Mbd1 which is occupied by CTCF and cohesin, and an adjacent upstream, as a negative control (NC) (Figs. 5b and S4b). d Purification of biotinylated <t>dCas9</t> and Western blot indicated that Matr3 interacted with the putative element (Mbd1) but not with the upstream control (NC). Relative enrichment of Matr3 protein at the putative element to the upstream control was quantified from three independent experiments. e Radial location of the Mbd1 gene was observed by 3D DNA FISH. For quantification, the nuclear radius was divided into five shells and the number of signals in each shell was counted. The graph is the result of 2–3 independent experiments and the change was significant under a two-sided t test ( p = 0.006; Cohen’s d = 0.32). f Snapshot of Hi-C data at the Mbd1 locus showing compartments in parental and Matr3 KO cells and the difference in insulation score (parental in green and Matr3 KO in magenta). g , h Deletion of CTCF/cohesin binding region (shaded box in Fig. 5b) by CRISPR/Cas9 reduced Mbd1 expression. RNA ( g ) and protein ( h ) levels were examined by RT-PCR ( n = 3) and Western blotting, and compared to Mbd1 KO cells. β-actin was used for a Western blot control. Data were the result of 2–3 independent experiments and error bars represent mean ± s.d. Source data are provided as a Source Data file.
Plvx Mcmv Zsgreen1, supplied by Biowit Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+vector+plvx+ires+zsgreen1/plvx+mcmv+zsgreen1/pmc03851560-125-12-16
Average 90 stars, based on 1 article reviews
plvx-mcmv-zsgreen1 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
vectors plvx-ires-zsgreen1  (PSICOR Inc)
90
PSICOR Inc vectors plvx-ires-zsgreen1
a Reduced expression level of Mbd1 was revealed by RNA-seq from two independent experiments and Western blot. β-actin was used for a Western blotting control. b Chromatin occupancy of CTCF and Rad21 in parental and Matr3 KO cells near Mbd1 locus is shown. c CAPTURE experiment was performed using sgRNAs directed to a putative cis-regulatory element near Mbd1 which is occupied by CTCF and cohesin, and an adjacent upstream, as a negative control (NC) (Figs. 5b and S4b). d Purification of biotinylated <t>dCas9</t> and Western blot indicated that Matr3 interacted with the putative element (Mbd1) but not with the upstream control (NC). Relative enrichment of Matr3 protein at the putative element to the upstream control was quantified from three independent experiments. e Radial location of the Mbd1 gene was observed by 3D DNA FISH. For quantification, the nuclear radius was divided into five shells and the number of signals in each shell was counted. The graph is the result of 2–3 independent experiments and the change was significant under a two-sided t test ( p = 0.006; Cohen’s d = 0.32). f Snapshot of Hi-C data at the Mbd1 locus showing compartments in parental and Matr3 KO cells and the difference in insulation score (parental in green and Matr3 KO in magenta). g , h Deletion of CTCF/cohesin binding region (shaded box in Fig. 5b) by CRISPR/Cas9 reduced Mbd1 expression. RNA ( g ) and protein ( h ) levels were examined by RT-PCR ( n = 3) and Western blotting, and compared to Mbd1 KO cells. β-actin was used for a Western blot control. Data were the result of 2–3 independent experiments and error bars represent mean ± s.d. Source data are provided as a Source Data file.
Vectors Plvx Ires Zsgreen1, supplied by PSICOR Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+vector+plvx+ires+zsgreen1/vectors+plvx+ires+zsgreen1/pmc11655532-300-3-4
Average 90 stars, based on 1 article reviews
vectors plvx-ires-zsgreen1 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
zsgreen1 fragment  (Addgene inc)
93
Addgene inc zsgreen1 fragment
a Reduced expression level of Mbd1 was revealed by RNA-seq from two independent experiments and Western blot. β-actin was used for a Western blotting control. b Chromatin occupancy of CTCF and Rad21 in parental and Matr3 KO cells near Mbd1 locus is shown. c CAPTURE experiment was performed using sgRNAs directed to a putative cis-regulatory element near Mbd1 which is occupied by CTCF and cohesin, and an adjacent upstream, as a negative control (NC) (Figs. 5b and S4b). d Purification of biotinylated <t>dCas9</t> and Western blot indicated that Matr3 interacted with the putative element (Mbd1) but not with the upstream control (NC). Relative enrichment of Matr3 protein at the putative element to the upstream control was quantified from three independent experiments. e Radial location of the Mbd1 gene was observed by 3D DNA FISH. For quantification, the nuclear radius was divided into five shells and the number of signals in each shell was counted. The graph is the result of 2–3 independent experiments and the change was significant under a two-sided t test ( p = 0.006; Cohen’s d = 0.32). f Snapshot of Hi-C data at the Mbd1 locus showing compartments in parental and Matr3 KO cells and the difference in insulation score (parental in green and Matr3 KO in magenta). g , h Deletion of CTCF/cohesin binding region (shaded box in Fig. 5b) by CRISPR/Cas9 reduced Mbd1 expression. RNA ( g ) and protein ( h ) levels were examined by RT-PCR ( n = 3) and Western blotting, and compared to Mbd1 KO cells. β-actin was used for a Western blot control. Data were the result of 2–3 independent experiments and error bars represent mean ± s.d. Source data are provided as a Source Data file.
Zsgreen1 Fragment, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+vector+plvx+ires+zsgreen1/pCAG-roxSTOProx-ZsGreen+(Plasmid+%2351274)/bio_rxiv__2023__06__17__545314-251-2-12
Average 93 stars, based on 1 article reviews
zsgreen1 fragment - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
lentiviral vector plvx ires zsgreen1  (Addgene inc)
93
Addgene inc lentiviral vector plvx ires zsgreen1
a Reduced expression level of Mbd1 was revealed by RNA-seq from two independent experiments and Western blot. β-actin was used for a Western blotting control. b Chromatin occupancy of CTCF and Rad21 in parental and Matr3 KO cells near Mbd1 locus is shown. c CAPTURE experiment was performed using sgRNAs directed to a putative cis-regulatory element near Mbd1 which is occupied by CTCF and cohesin, and an adjacent upstream, as a negative control (NC) (Figs. 5b and S4b). d Purification of biotinylated <t>dCas9</t> and Western blot indicated that Matr3 interacted with the putative element (Mbd1) but not with the upstream control (NC). Relative enrichment of Matr3 protein at the putative element to the upstream control was quantified from three independent experiments. e Radial location of the Mbd1 gene was observed by 3D DNA FISH. For quantification, the nuclear radius was divided into five shells and the number of signals in each shell was counted. The graph is the result of 2–3 independent experiments and the change was significant under a two-sided t test ( p = 0.006; Cohen’s d = 0.32). f Snapshot of Hi-C data at the Mbd1 locus showing compartments in parental and Matr3 KO cells and the difference in insulation score (parental in green and Matr3 KO in magenta). g , h Deletion of CTCF/cohesin binding region (shaded box in Fig. 5b) by CRISPR/Cas9 reduced Mbd1 expression. RNA ( g ) and protein ( h ) levels were examined by RT-PCR ( n = 3) and Western blotting, and compared to Mbd1 KO cells. β-actin was used for a Western blot control. Data were the result of 2–3 independent experiments and error bars represent mean ± s.d. Source data are provided as a Source Data file.
Lentiviral Vector Plvx Ires Zsgreen1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+vector+plvx+ires+zsgreen1/ZsGreen1-N1+(Plasmid+%2354702)/pm38438894-57-7-20
Average 93 stars, based on 1 article reviews
lentiviral vector plvx ires zsgreen1 - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
cmv mcs ef1 zsgreen1 pgk puro vector  (Addgene inc)
86
Addgene inc cmv mcs ef1 zsgreen1 pgk puro vector
a Reduced expression level of Mbd1 was revealed by RNA-seq from two independent experiments and Western blot. β-actin was used for a Western blotting control. b Chromatin occupancy of CTCF and Rad21 in parental and Matr3 KO cells near Mbd1 locus is shown. c CAPTURE experiment was performed using sgRNAs directed to a putative cis-regulatory element near Mbd1 which is occupied by CTCF and cohesin, and an adjacent upstream, as a negative control (NC) (Figs. 5b and S4b). d Purification of biotinylated <t>dCas9</t> and Western blot indicated that Matr3 interacted with the putative element (Mbd1) but not with the upstream control (NC). Relative enrichment of Matr3 protein at the putative element to the upstream control was quantified from three independent experiments. e Radial location of the Mbd1 gene was observed by 3D DNA FISH. For quantification, the nuclear radius was divided into five shells and the number of signals in each shell was counted. The graph is the result of 2–3 independent experiments and the change was significant under a two-sided t test ( p = 0.006; Cohen’s d = 0.32). f Snapshot of Hi-C data at the Mbd1 locus showing compartments in parental and Matr3 KO cells and the difference in insulation score (parental in green and Matr3 KO in magenta). g , h Deletion of CTCF/cohesin binding region (shaded box in Fig. 5b) by CRISPR/Cas9 reduced Mbd1 expression. RNA ( g ) and protein ( h ) levels were examined by RT-PCR ( n = 3) and Western blotting, and compared to Mbd1 KO cells. β-actin was used for a Western blot control. Data were the result of 2–3 independent experiments and error bars represent mean ± s.d. Source data are provided as a Source Data file.
Cmv Mcs Ef1 Zsgreen1 Pgk Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+vector+plvx+ires+zsgreen1/ZsGreen1-CytERM-N-17+(Plasmid+%2356297)/pm29741668-79-12-15
Average 86 stars, based on 1 article reviews
cmv mcs ef1 zsgreen1 pgk puro vector - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier
lenti sgrna vectors  (Addgene inc)
93
Addgene inc lenti sgrna vectors
a Reduced expression level of Mbd1 was revealed by RNA-seq from two independent experiments and Western blot. β-actin was used for a Western blotting control. b Chromatin occupancy of CTCF and Rad21 in parental and Matr3 KO cells near Mbd1 locus is shown. c CAPTURE experiment was performed using sgRNAs directed to a putative cis-regulatory element near Mbd1 which is occupied by CTCF and cohesin, and an adjacent upstream, as a negative control (NC) (Figs. 5b and S4b). d Purification of biotinylated <t>dCas9</t> and Western blot indicated that Matr3 interacted with the putative element (Mbd1) but not with the upstream control (NC). Relative enrichment of Matr3 protein at the putative element to the upstream control was quantified from three independent experiments. e Radial location of the Mbd1 gene was observed by 3D DNA FISH. For quantification, the nuclear radius was divided into five shells and the number of signals in each shell was counted. The graph is the result of 2–3 independent experiments and the change was significant under a two-sided t test ( p = 0.006; Cohen’s d = 0.32). f Snapshot of Hi-C data at the Mbd1 locus showing compartments in parental and Matr3 KO cells and the difference in insulation score (parental in green and Matr3 KO in magenta). g , h Deletion of CTCF/cohesin binding region (shaded box in Fig. 5b) by CRISPR/Cas9 reduced Mbd1 expression. RNA ( g ) and protein ( h ) levels were examined by RT-PCR ( n = 3) and Western blotting, and compared to Mbd1 KO cells. β-actin was used for a Western blot control. Data were the result of 2–3 independent experiments and error bars represent mean ± s.d. Source data are provided as a Source Data file.
Lenti Sgrna Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+vector+plvx+ires+zsgreen1/Lenti_sgRNA(MS2)_zsGreen1+(Plasmid+%23138463)/pm34133940-618-0-8
Average 93 stars, based on 1 article reviews
lenti sgrna vectors - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
plvx ires zsgreen1 shuttle vector plasmid  (ATCC)
99
ATCC plvx ires zsgreen1 shuttle vector plasmid
a Reduced expression level of Mbd1 was revealed by RNA-seq from two independent experiments and Western blot. β-actin was used for a Western blotting control. b Chromatin occupancy of CTCF and Rad21 in parental and Matr3 KO cells near Mbd1 locus is shown. c CAPTURE experiment was performed using sgRNAs directed to a putative cis-regulatory element near Mbd1 which is occupied by CTCF and cohesin, and an adjacent upstream, as a negative control (NC) (Figs. 5b and S4b). d Purification of biotinylated <t>dCas9</t> and Western blot indicated that Matr3 interacted with the putative element (Mbd1) but not with the upstream control (NC). Relative enrichment of Matr3 protein at the putative element to the upstream control was quantified from three independent experiments. e Radial location of the Mbd1 gene was observed by 3D DNA FISH. For quantification, the nuclear radius was divided into five shells and the number of signals in each shell was counted. The graph is the result of 2–3 independent experiments and the change was significant under a two-sided t test ( p = 0.006; Cohen’s d = 0.32). f Snapshot of Hi-C data at the Mbd1 locus showing compartments in parental and Matr3 KO cells and the difference in insulation score (parental in green and Matr3 KO in magenta). g , h Deletion of CTCF/cohesin binding region (shaded box in Fig. 5b) by CRISPR/Cas9 reduced Mbd1 expression. RNA ( g ) and protein ( h ) levels were examined by RT-PCR ( n = 3) and Western blotting, and compared to Mbd1 KO cells. β-actin was used for a Western blot control. Data were the result of 2–3 independent experiments and error bars represent mean ± s.d. Source data are provided as a Source Data file.
Plvx Ires Zsgreen1 Shuttle Vector Plasmid, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+vector+plvx+ires+zsgreen1/Plasmid/pmc09425432-52-1-16
Average 99 stars, based on 1 article reviews
plvx ires zsgreen1 shuttle vector plasmid - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
pmr zsgreen1 vector  (TaKaRa)
94
TaKaRa pmr zsgreen1 vector
a Reduced expression level of Mbd1 was revealed by RNA-seq from two independent experiments and Western blot. β-actin was used for a Western blotting control. b Chromatin occupancy of CTCF and Rad21 in parental and Matr3 KO cells near Mbd1 locus is shown. c CAPTURE experiment was performed using sgRNAs directed to a putative cis-regulatory element near Mbd1 which is occupied by CTCF and cohesin, and an adjacent upstream, as a negative control (NC) (Figs. 5b and S4b). d Purification of biotinylated <t>dCas9</t> and Western blot indicated that Matr3 interacted with the putative element (Mbd1) but not with the upstream control (NC). Relative enrichment of Matr3 protein at the putative element to the upstream control was quantified from three independent experiments. e Radial location of the Mbd1 gene was observed by 3D DNA FISH. For quantification, the nuclear radius was divided into five shells and the number of signals in each shell was counted. The graph is the result of 2–3 independent experiments and the change was significant under a two-sided t test ( p = 0.006; Cohen’s d = 0.32). f Snapshot of Hi-C data at the Mbd1 locus showing compartments in parental and Matr3 KO cells and the difference in insulation score (parental in green and Matr3 KO in magenta). g , h Deletion of CTCF/cohesin binding region (shaded box in Fig. 5b) by CRISPR/Cas9 reduced Mbd1 expression. RNA ( g ) and protein ( h ) levels were examined by RT-PCR ( n = 3) and Western blotting, and compared to Mbd1 KO cells. β-actin was used for a Western blot control. Data were the result of 2–3 independent experiments and error bars represent mean ± s.d. Source data are provided as a Source Data file.
Pmr Zsgreen1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+vector+plvx+ires+zsgreen1/pmR-ZsGreen1+Vector/10__1016_slash_j__cej__2022__137637-53-14-16
Average 94 stars, based on 1 article reviews
pmr zsgreen1 vector - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
plvx ires zsgreen1 tim 3 plasmid  (Thermo Fisher)
96
Thermo Fisher plvx ires zsgreen1 tim 3 plasmid
a Reduced expression level of Mbd1 was revealed by RNA-seq from two independent experiments and Western blot. β-actin was used for a Western blotting control. b Chromatin occupancy of CTCF and Rad21 in parental and Matr3 KO cells near Mbd1 locus is shown. c CAPTURE experiment was performed using sgRNAs directed to a putative cis-regulatory element near Mbd1 which is occupied by CTCF and cohesin, and an adjacent upstream, as a negative control (NC) (Figs. 5b and S4b). d Purification of biotinylated <t>dCas9</t> and Western blot indicated that Matr3 interacted with the putative element (Mbd1) but not with the upstream control (NC). Relative enrichment of Matr3 protein at the putative element to the upstream control was quantified from three independent experiments. e Radial location of the Mbd1 gene was observed by 3D DNA FISH. For quantification, the nuclear radius was divided into five shells and the number of signals in each shell was counted. The graph is the result of 2–3 independent experiments and the change was significant under a two-sided t test ( p = 0.006; Cohen’s d = 0.32). f Snapshot of Hi-C data at the Mbd1 locus showing compartments in parental and Matr3 KO cells and the difference in insulation score (parental in green and Matr3 KO in magenta). g , h Deletion of CTCF/cohesin binding region (shaded box in Fig. 5b) by CRISPR/Cas9 reduced Mbd1 expression. RNA ( g ) and protein ( h ) levels were examined by RT-PCR ( n = 3) and Western blotting, and compared to Mbd1 KO cells. β-actin was used for a Western blot control. Data were the result of 2–3 independent experiments and error bars represent mean ± s.d. Source data are provided as a Source Data file.
Plvx Ires Zsgreen1 Tim 3 Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+vector+plvx+ires+zsgreen1/TIMOLOL+MALEATE/pm28339054-41-8-20
Average 96 stars, based on 1 article reviews
plvx ires zsgreen1 tim 3 plasmid - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

Image Search Results


a Reduced expression level of Mbd1 was revealed by RNA-seq from two independent experiments and Western blot. β-actin was used for a Western blotting control. b Chromatin occupancy of CTCF and Rad21 in parental and Matr3 KO cells near Mbd1 locus is shown. c CAPTURE experiment was performed using sgRNAs directed to a putative cis-regulatory element near Mbd1 which is occupied by CTCF and cohesin, and an adjacent upstream, as a negative control (NC) (Figs. 5b and S4b). d Purification of biotinylated dCas9 and Western blot indicated that Matr3 interacted with the putative element (Mbd1) but not with the upstream control (NC). Relative enrichment of Matr3 protein at the putative element to the upstream control was quantified from three independent experiments. e Radial location of the Mbd1 gene was observed by 3D DNA FISH. For quantification, the nuclear radius was divided into five shells and the number of signals in each shell was counted. The graph is the result of 2–3 independent experiments and the change was significant under a two-sided t test ( p = 0.006; Cohen’s d = 0.32). f Snapshot of Hi-C data at the Mbd1 locus showing compartments in parental and Matr3 KO cells and the difference in insulation score (parental in green and Matr3 KO in magenta). g , h Deletion of CTCF/cohesin binding region (shaded box in Fig. 5b) by CRISPR/Cas9 reduced Mbd1 expression. RNA ( g ) and protein ( h ) levels were examined by RT-PCR ( n = 3) and Western blotting, and compared to Mbd1 KO cells. β-actin was used for a Western blot control. Data were the result of 2–3 independent experiments and error bars represent mean ± s.d. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture

doi: 10.1038/s41467-021-26574-4

Figure Lengend Snippet: a Reduced expression level of Mbd1 was revealed by RNA-seq from two independent experiments and Western blot. β-actin was used for a Western blotting control. b Chromatin occupancy of CTCF and Rad21 in parental and Matr3 KO cells near Mbd1 locus is shown. c CAPTURE experiment was performed using sgRNAs directed to a putative cis-regulatory element near Mbd1 which is occupied by CTCF and cohesin, and an adjacent upstream, as a negative control (NC) (Figs. 5b and S4b). d Purification of biotinylated dCas9 and Western blot indicated that Matr3 interacted with the putative element (Mbd1) but not with the upstream control (NC). Relative enrichment of Matr3 protein at the putative element to the upstream control was quantified from three independent experiments. e Radial location of the Mbd1 gene was observed by 3D DNA FISH. For quantification, the nuclear radius was divided into five shells and the number of signals in each shell was counted. The graph is the result of 2–3 independent experiments and the change was significant under a two-sided t test ( p = 0.006; Cohen’s d = 0.32). f Snapshot of Hi-C data at the Mbd1 locus showing compartments in parental and Matr3 KO cells and the difference in insulation score (parental in green and Matr3 KO in magenta). g , h Deletion of CTCF/cohesin binding region (shaded box in Fig. 5b) by CRISPR/Cas9 reduced Mbd1 expression. RNA ( g ) and protein ( h ) levels were examined by RT-PCR ( n = 3) and Western blotting, and compared to Mbd1 KO cells. β-actin was used for a Western blot control. Data were the result of 2–3 independent experiments and error bars represent mean ± s.d. Source data are provided as a Source Data file.

Article Snippet: To generate the pEF1a-dCas9-CBio-IRES-zsGreen1-puro vector, the dCas9-CBio-IRES-zsGreen1 fragment was amplified from pLVX-EF1a-dCas9-CBio-IRES-zsGreen1 (Addgene #138418) as the template and cloned into XbaI and SmaI double-digested pEF1a-FB-dCas9-puro vector (Addgene #100547) by In-Fusion HD Cloning Kit (Clontech).

Techniques: Expressing, RNA Sequencing Assay, Western Blot, Negative Control, Purification, Hi-C, Binding Assay, CRISPR, Reverse Transcription Polymerase Chain Reaction